Forensics/DNA

Speciality cards for space- and time-saving collection, archival storage, and isolation of genomic DNA from a variety of sample material. Originally Whatman.

• Capture nucleic acid in one easy step - simply apply blood or
  tissue material to the card
• Captured nucleic acid is ready for downstream applications in
  less than 30 mins.
• Genomic DNA collected on FTA^® Cards is stable for years at
  room temperature
• Suitable for virtually any cell type
• Indicating FTA^® Cards change colour upon sample application in order to facilitate handling of colourless samples

Simply apply your sample to the FTA^® Card. Liquids are pipetted or trickled, tissue samples are pressed onto the surface. Cell membranes and organelles are automatically lysed and the released nucleic acids are entrapped in the fibres of the matrix (see figure). The nucleic acids remain immobilized and are stabilized for transport, immediate processing or long-term storage. Libraries and species DNA collections can be stored simply in a folder; single DNA samples can be filed in your lab manual. FTA^® Cards facilitate sample collection in remote locations and simplify sample transport, thus, you can collect samples in the field without worrying about refrigeration.
Captured nucleic acids are used for downstream experiments by simply taking a small punch from the FTA^® Card, wash with FTA^® Purification Reagent and rinse with TE buffer (Roti^®-Stock 100 x TE Buffer, Art. No. 1052.1). DNA on the washed punch is ready for use in a variety of applications given below. Genomic DNA stored on FTA^® Cards at room temperature for over 14 years (and counting) has been successfully amplified by PCR. For RNA, being more sensitive than DNA, we recommend rapid analysis after only few weeks' storage at room temperature.

FTA^® Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidative and UV damage. FTA^® Cards rapidly inactivate organisms, including blood borne pathogens, and prevent the growth of bacteria and other microorganisms. Each card can be used for archival storage or multiple downstream applications of 1-2 (MINI) and 1-4 (CLASSIC) independent samples.

Suitable for blood, cultivated cells, buccal cells, plant material, bacteria, microorganisms, tissue and tissue homogenisations, virions, M13 plaques and more.

Applications: storage of genomic DNA samples and libraries, collection of nucleic acid from animal tissue or cell cultures, or from plants or microorganisms during in-field-experiments.

Downstream-Experiments: e. g. PCR, real-time PCR, SNP and STR analysis, transgenetic identification, whole genome amplification, HLA typing, and genotyping in forensics, molecular biology, food and agriculture testing, drug discovery, genomics, species identification, and more.

Speciality cards for space- and time-saving archival storage and isolation of Plasmid and BAC DNA. Originally Whatman.

• Capture DNA in one easy step by simply applying the samples (recombinant bacteria, glycerol stocks etc.) to the card
• Captured nucleic acid is easily accessible for downstream applications
• DNA collected on FTA^® CloneSaver Cards is stable for years at
  room temperature
• Store up to 96 clones on each card
• Standard 96well plate format and compatible with multi-channel pipettors

Apply few µl bacterial culture, resuspended colony or glycerol stock. Cells are lysed and plasmid or BAC DNA is stabilized for long-term storage or immediate processing. Captured nucleic acids are used for downstream experiments without the need for culture regrowth and plasmid purification. Simply take a small punch from the FTA^® Card and wash with TE buffer (Roti^®-Stock 100x TE Buffer, Art. No. 1052.1). DNA on the washed punch is ready for elution or for direct use in PCR, transformation, amplification by rolling circle followed by sequencing, and so on.

Pipette NOT included !

Uni-Core Punch is a disposable punch which has a polished steel tip that is case-hardened and can be sterilized. Each tip provides up to 500 punches before disposal. Uni-Core Punch is available in diameters from 1,0 to 6,0 mm for a variety of sampling needs. It is housed in a plastic handle and disposable for contamination control. Stainless steel cutting edges enable manual coring, retrieval, and controlled ejection of the FTA punch. Manual punching is preferred for laboratories with low sample volumes punching 1 to 50 samples per day.

Very easy application: By pressing the Micro tip down a tiny slice is punched from the FTA^® card. This DNA containing sample is then washed and directly applied to downstream experiments.

Two cutting mats are included. Colour of the punches supplied may differ to that shown in the picture.

For transporting or storing FTA^® Cards. Originally Whatman.

Seven laminated layers protect the card from exposure to gas or liquid contamination. Tamper-evident seal maintains sample security. Outer paper surface for labelling or writing.
CloneSaver pouches only: with additional resealable zip-lock closure.